NIH/3T3-EGFP cell line

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NIH/3T3-EGFP Order

NIH/3T3-EGFP cell line
Catalog#
YC-A013-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NIH/3T3-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
NIH/3T3-EGFP
Cell Morphology
Fibroblast-like, adherent
Passage ratio
1:3~1:5
Species
Mouse
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NIH/3T3		 STR Info (Cell bank)
Cell Line:NIH 3T3
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 19.320.319.320.3
6-7 12.012.0
19-2 10.011.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.010.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.015.017.0
17-2 13.014.013.014.0
12-1 20.020.0
5-5 14.015.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the NIH/3T3 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NIH/3T3-EGFP cell line that can maintain long-term, stable and high expression.
NIH/3T3-EGFP cell line
NIH/3T3-EGFP
Catalog#
YC-A013-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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